Smyte 19-Final program

  

 

 

Friday September 14th, 2001

14:00 - 18:00

Registration

19:30

Opening of the Meeting

20:00

Nico van Uden lecture: Novel Ion Transporters of the Endomembrane System: from Yeast to Human
R. Rao

21:00

Welcome Dinner - A taste of Cretan Music

 

Saturday September 15th, 2001

 

 

 

Session 1: ATPases and Ion Transport

                                                (Chair: R. L. Brandao)

 

9:00 - 9:20

New insights into the regulation of the yeast Pma1 H+-ATPase by glucose

M. Miranda, J.P. Pardo, K.E. Allen and C.W. Slayman

9:20 - 9:40

Regulation of the plasma membrane ATPase in Saccharomyces cerevisiae cells
R. L. Brandao, I.M. Castro, M. A. de Souza Andrade

9:40 - 10:00

A Role for Tok1 in K+ uptake in Yeast
A. Bertl, G. Natura and H. Bihler

10:00 - 10:20

Low-affinity potassium uptake by Saccharomyces cerevisiae is mediated by Nsc1, a calcium-blocked non-specific cation channel
H. Bihler, C.L. Slayman and A. Bertl

10:20 - 10:40

Physiological function of potassium channels in yeast
J. Ludwig, B. Kirberg and H. Lichtenberg

10:40 - 11:00

Electrophoretic properties of the Trk transporters in Saccharomyces cereviciae
T. Kuroda, A. Rivetta, G. Zeng and C.L. Slayman

11:00 - 11:20

Mac1p modulation by copper and protein interactions in Saccharomyces cerevisiae
A. Voutsina, G.S. Fragiadakis and D. Alexandraki

11:20 - 11:35

Coffee Break

11:35 - 11:55

The Mrs2 family of putative mitochondrial magnsium transporters
J. Gregan, G. Zsurka, M. Kolisek, M. Schweigel, R.J. Schweyen

11:55 - 12:15

Yeast proteins involved in Mg2+ homeostasis mediate rapid changes in the free Mg2+ concentration
A. Graschopf, M. Kolisek, J. A. Stadler, M. Schweigel and R. J. Schweyen

12:15 - 12:35

The Yeast Plasma Membrane Protein Alr1 Controls Magnesium Homeostasis and Is Subject to Magnesium dependent Control of Its Synthesis and Degradation
J.A. Stadler, A. Graschopf, M.K. Hoellerer and R.J. Schweyen

12:35 - 12:55

Transport properties of two subfamilies of yeast alkali-metal-cation/H+ antiporters
O. Kinclova, S. Potier and H. Sychrova

13:00 - 14:00

Lunch

14:00 –16:15

Visit to Archeological Museum of Chania (optional)

 

 

Session 2: Purine, Amino Acid and Sugar Transporters

                                (Chair: J.R. Mattoon)

 

16:30 - 16:50

A novel purine transporter is encoded by the azgA gene of Aspergillus nidulans

G. Cecchetto, S. Amillis, C. Scazzocchio, G. Diallinas and C. Drevet

16:50 - 17:10

Aspergillus nidulans purine transporters are differentially regulated in response to development, different nitrogen sources and the presence of purines

S. Amillis, G. Cecchetto, C. Scazzocchio and G. Diallinas

17:10 - 17:30

Threonine transport in Saccharomyces cerevisiae
J. R. Mattoon, M. Place, M. Hensley, C. Williams, D. Ward, M. Chianelli, E. Ramos and C.A. Stella

17:30 - 17:50

Functional analysis of Ghtp1 and Ghtp3 transporters of Schizosaccharomyces pombe
M. Hoefer, N. Radovanovic, M Schmitt, S. Hafkenmeyer and H. Lichtenberg

17:50 - 18:10

Carbon source regulation of FSY1, a gene encoding a fructose/H+ symporter in Saccharomyces cerevisiae
H. Rodrigues de Sousa, P. Goncalves and I. Spencer-Martins

18:10 - 18:30

Partial purification of the lactate carrier protein of the yeast C. utilis
F. Baltazar, F. Cassio and C. Leao

18:30 -.18:50

Functional characterization of a putative glucose transporter of Trichoderma reesei

A. S. P. Ramos, F. Chambergo and H. El-Dorry

18:50 – 19:10

Heme involvement in the control of sterol uptake and esterification in yeast

L. Hronska, M. Valachovic, V. Klobucnikova and I. Hapala

19.10 - 19.25

Coffee Break

 

Session 3: Structure-Function Relationships

                                (Chair: C.W. Slayman)

 

19:25 - 19:45

Structure-Function relationship in membrane segment 8 of the yeast PMA1-ATPase

G. Guerra, V. V. Petrov, K. E. Allen, M. Miranda and C. W. Slayman

 

19:45 - 20:05

Structure-Function analysis of Tok1

G. Natura, H. Bihler and A. Bertl

 

20:05 - 20:25

Genetic alteration of the specificity and kinetics of the uric-acid xanthine transporter in Aspergillus nidulans

M. koukaki, S. Amillis and G. Diallinas

 

20:30 - 21:00

General Discussion of Sessions 1-3

 

21:15

Night Visit (Dinner) to Chania

 

 

 

Sanday September 16th, 2001

 

Session 4: Regulation of Expression-Post-Translational Control and Topogenesis

                                (Chair: C. Scazzocchio)

 

8:50 - 9:25

Lecture: Nucleosome remodelling and transcriptional activation in Saccharomyces cerevisiae

G. Thireos, P. Syntichaki and I. Topalidou

9:25 - 10:00

Lecture: Integration of multiple regulatory systems in the control of expression of the proline transporter of Aspergillus nidulans

C. Scazzocchio

10:00 - 10:20

ER associated degradation of amino acid permeases in shr3 null mutant yeast

C. F. Gilstring and P.O. Ljungdahl

10:20 - 10:40

Checkpoints in the yeast sectretory pathway indicated by atypical biogenesis of the plasma membrane H+-ATPase mutant, pma1-G381A

A. B. Mason, T. Ferreira, K.E. Allen, M. Pypaert and C.W. Slayman

10:40 - 11:00

Characterization of a gene possibly involved in the control of permease functionality in the membrane of Aspergillus nidulans
L. Harispe, C. Portela, C. Scazzocchio and L. Gorfinkiel

11:00 - 11:20

Mutations affecting pleitropically amino acid transport systems in
Aspergillus nidulans

A. Apostolaki, C. Drevet and C. Scazzocchio

11:20 - 11:35

Coffee Break

 

 

Session 5: Novel Approaches

                                (Chair: H. Lichtenberg)

 

11:35 - 11:55

Roles of Phospholipids in Transport Protein Activity

I. Robl, W. Tanner and M. Opekarova

11:55 - 12:15

Lysosomotropic N,N-dimethyl aminoesters and their quaternary ammonium salts as plasma membrane and mitochondrial ATPase inhibitors

E. Oblak, T. M. Lachowicz, J. Luczynski, and S. Witek

12:15 - 12:35

Vacuolar pH changes caused by selected lysosomotropic drugs in different yeast species
A. Krasowska, L. Chmielewska, K. Siegler, J. Luczynski and S. Witek

12:35 - 12:55

Fluorescence based assay for detection of genotoxic noxious emission
H. Lichtenberg, P. Schwanewilm, M. Schmitt and J. Ludwig

12:55 - 13:15

Functional expression and cellular localization of a green fluorescent protein-tagged proline transporter in Aspergillus nidulans

S. N. Tavoularis, C. Scazzocchio and V. Sophianopoulou

13:15 - 13:35

Role for an oxidase alternative to the cytochromic chain in yeasts
A. Veiga, J. D. Arrabaca and M. C. Lureido-Dias

13:35 - 13:55

Engineering of gene expression in baker's yeast through the application of a novel promoter fusion cassette system
M. Spaans, S. Donkers, Th. Petit and Han de Winde

14:00

Lunch

 

Session 6: Multidrug Resistance and Stress

                                (Chair: J. Kolarov)

 

17:00 - 17:20

The First Multidrug ABC Transporter in Kluyveromyces lactis

Y. Gbelska, M. Takacova, P. Sklenar, K.D. Breunig and J. Subik

17:20 - 17:40

Yeast PDR pumps as affected by growth and physiological conditions
K. Sigler, D. Gaskova, R. Cadek and K. Chladkova

17:40 - 18:00

Triggering yeast to apoptosis

L. Sabova, D. Poliakova, I. Kissova, V. Pevala and J. Kolarov

18:00 - 18:20

The role of oxygen radicals in bax induced apoptosis in Kluyveromyces lactis

L. Sabova, D. Poliakova, J. Kolarov

18:20 - 18:40

The sensitivity of S. cerevisiae cells to keller toxins k1 and k2 is influenced by alterations of plasma membrane

H. Flegelova, Novotna D. and Janderova B.

18:40 - 19:00

Sodium sequestration in the vacuole of Debaryomyces hansenii
V. Montiel, M. Rosas, A. Almagro and J. Ramos

19:00 - 19:20

Coffee break

19:20- 19:50

General Discussion of Sessions 4-6

20.00

Visit-Dinner at Vamos traditional village

 

 

Monday September 17th, 2001

 

Session 7: Paradigms of Transporter Studies from Bacteria, Protozoa, Plants

                     and Metazoans-Fungi As Model Systems

                                                (Chair: G. Diallinas)

 

9:00 - 9:40

Lecture: Ptotozoan nucleobase transporters: substrate recognition motifs reveal selective uptake of purine antimetabolites

L. JM. McMurray, D. Candlish, S. E. Coutts and H. P. de Koning

9:40 - 10:00

Bacterial protein translocase: molecular mechanism and energetics

S. Karamanou, C. Baud, E., Vrontou, G. Sianidis and A. Economou

10:00 - 10:20

The Na+/I- symporter expression in mammary gland during lactation and in breast cancer

U. H. Tazebay, I. L. Wapnir, O. Levy, L. S. Zuckier, R. G. Pestell, and N. Carrasco.

10:20 - 10:40

Expression of plant and human nuclobase/ascorbate transporters in Aspergillus nidulans

E. Argyrou, G. Diallinas, L. Gorfinkiel, E. Holtzman and V. Sophianopoulou

10:40 - 11:00

The OHS family of transporters

S. Frillingos, M. Sabin-Toth and H. R. Kaback

11:00 - 11:10

Coffee break

 

 

 

 

 

 

Session 8: Ammonia Sensing

                                (Chair: Z. Palkova)

 

11:10 - 11:30

Ammonia effect on yeast colonies: New putative ammonium exporters
M. Ricicova, Z. Palkova, F. Devaux, C. Jacq and A. Goffeau

11:30 - 11:50

Ammonia effect on yeast colonies: Model of cellular changes revealed by cytochemical, genetic and microarray analyses
Z. Palkova, F. Devaux, M. Ricicova and C. Jacq

11:50 - 12:10

Ammonia effect on yeast colonies: Ammonia induces changes of amino acid distribution between vacuole and cytoplasm
L. Minarikova and Z. Palkova

12.10 -:13.10

Discussion of Sessions 7-8 and General Discussion

13:30.-.20:00

Visit to Falassarna for swimming and lunch

21.00
Closing of the Meeting

 

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